罗 丹¹，陈 丹¹，白曦晨²，向极钎³，樊建元⁴，刘 卫^2，5*

（1.武汉百思凯瑞生物科技有限公司，湖北武汉 430075；

2.华中科技大学生命科学与技术学院，湖北武汉 430074；

3.恩施州农业科学院，湖北恩施 445099；

4.恩施思慧益臣生态农业发展有限公司，湖北恩施 445099；

5.华中科技大学国家纳米药物工程技术研究中心，湖北武汉 430075）

摘要：从细胞免疫、体液免疫、非特异性免疫三方面研究富硒青钱柳-蛹虫草复方对C57小鼠免疫功能的影响及抗氧化作用。将C57小鼠随机分为阴性对照组、阳药组、富硒青钱柳-蛹虫草复方低、中、高剂量组。分别进行Con A诱导脾淋巴细胞体外增殖实验、血清溶血素实验、巨噬细胞吞噬鸡红细胞实验及自然杀伤细胞NK细胞活性实验，评价富硒青钱柳-蛹虫草复方对小鼠免疫调节功能的影响。此外，通过流式细胞仪测定不同浓度的富硒青钱柳-蛹虫草复方对NIT-1细胞胞内ROS水平的变化。结果显示，与阴性对照组比较，富硒青钱柳-蛹虫草复方对小鼠的体重、脾脏/体重比值、胸腺/体重比值无显著性影响（P＞0.05），低、中、高剂量组能显著促进Con A诱导的淋巴细胞增殖（P＜0.05），高剂量组对半数溶血值的增加有显著性影响（P＜0.05），且低、中、高各剂量组能显著增加吞噬指数和NK细胞活性（P＜0.05或P＜0.01）。且样品浓度为25～100 μg/mL时，胞内ROS水平与模型组比较显著降低（P＜0.01）。结果表明，富硒青钱柳-蛹虫草复方有效增强机体免疫力，具有显著的抗氧化功效。

关键词：富硒青钱柳；蛹虫草；C57小鼠；免疫调节；抗氧化

中图分类号：R285.5   文献标识码：A   文章编号：1674-506X（2021）04-0008-0007

Selenium-enriched Cyclocarya paliurus and Cordyceps militaris Compound Immunity and Antioxidant Experiment Research in Mice

LUO Dan¹, CHEN Dan¹, BAI Xi-chen², XIANG Ji-qian³, FAN Jian-yuan⁴, LIU Wei^2，5*

（1.Wuhan Bestcarrier Biotechnology Co., Ltd., Wuhan Hubei 430075, China;

2.College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan Hubei 430074, China;

3.Enshi Academy of Agricultural Sciences, Enshi Hubei 445099, China;

4.Enshi Savant ecological agriculture development Co., Ltd., Enshi Hubei 445099, China;

5.National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan Hubei 430075, China）

Abstract：From the cellular immunity, humoral immunity and nonspecific immune research Se-enriched Cyclocarya paliurus-Cordyceps militaris compound on the effect of C57 mice immune function and antioxidant function. The C57 mice were randomly divided into negative control group, positive drug group, Se-enriched Cyclocarya paliurus-Cordyceps militaris compound low, medium and high dose groups. Con A induced proliferation of spleen lymphocytes in vitro, serum hemolysin, macrophage phagocytosis of chicken red blood cells, and NK cell activity were conducted respectively to evaluate the effect of Se-enriched Cyclocarya paliurus-Cordyceps militaris compound on the immune regulatory function of mice. In addition, the changes of ROS levels in NIT-1 cells with different concentrations of Se-enriched Cyclocarya paliurus-Cordyceps militaris compound were measured by flow cytometry. The results showed that the Se-enriched Cyclocarya paliurus-Cordyceps militaris compound had no significant effect on the body weight, spleen/body weight ratio and thymus/body weight ratio of mice compared with the negative control group (P＞0.05), Con A induced lymphocyte proliferation was significantly promoted in low，medium and high dose groups (P＜0.05), the high dose group had a significant effect on the increase of half of the hemolysis value (P＜0.05). The phagocytosis index and NK cell activity were significantly increased in the low, medium and high dose groups (P＜0.05 or P＜0.01). And when the sample concentration was 25～100 μg/mL, the intracellular ROS level was significantly lower than that of the model group (P＜0.01). The results showed that the Se-enriched Cyclocarya paliurus- Cordyceps militaris compound has the ability to enhance immunity and the antioxidation.

Keywords：selenium-enriched Cyclocarya paliurus; Cordyceps militaris; C57 mice; immune regulation; antioxidant

doi：10.3969/j.issn.1674-506X.2021.04-002

收稿日期：2021-01-06

基金项目：恩施州特色资源应用技术与产品开发研究（湖北省科技支撑计划项目2013BEC048）。

作者简介：罗丹（1990-），硕士，助理研究员。研究方向：保健食品功效评价。

*通信作者

引文格式：罗丹,陈丹,白曦晨,等.富硒青钱柳-蛹虫草复方小鼠免疫调节及抗氧化实验研究[J].食品与发酵科技,2021,57(4):8-13,50.